Cyclic AMP induces a transient alkalinization in Dictyostelium
نویسندگان
چکیده
منابع مشابه
Cyclic AMP Receptors of Dictyostelium
P0005 When confronted with starvation, the social amoeba Dictyostelium discoideum survives by undergoing multicellular development and sporulation. The coordination of these processes is achieved in part through intercellular communication using secreted adenosine 30,50-cyclic monophosphate (cAMP) and a family of cell-surface cAMP receptors (cARs). The cARs are examples of G protein-coupled rec...
متن کاملChallenge with high concentrations of cyclic AMP induces transient changes in the cytosolic free calcium concentration in Dictyostelium discoideum.
Dictyostelium discoideum cells use cyclic AMP (cAMP) for chemotactic signaling as well as for differentiation. The precise regulation of the cytosolic Ca2+ concentration ([Ca2+]i) seems to play a key role for both processes. We performed single cell measurements of [Ca2+]i in amoebae that were starved in suspension for various times and scrape-loaded with the Ca2+ indicator fura-2. Stimulation ...
متن کاملIdentification and cyclic AMP-induced modification of the cyclic AMP receptor in Dictyostelium discoideum.
We have recently identified a cell surface cAMP-binding protein by specific photoaffinity labeling of intact Dictyostelium discoideum cells with 8-N3-[32P] cAMP. The major photolabeled protein appears as a doublet (Mr = 40,000-43,000) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis autoradiography. In this study, the doublet is shown to have the characteristics of the cAMP receptor...
متن کاملLocalization of cyclic-AMP receptors with acidosomes in Dictyostelium discoideum.
Earlier studies have shown that in Dictyostelium discoideum, a buoyant membrane fraction contained approximately 90% of the vacuolar proton pump (V-H(+)-ATPase) activity, leading to its designation acidosomes. It was proposed that acidosomes may be involved in endocytosis, specially in the acidification of endosomes. In this study we further investigated the putative function(s) of acidosomes. ...
متن کاملCyclic AMP stimulates accumulation of inositol trisphosphate in Dictyostelium.
Previous studies indicated that in Dictyostelium amoebae signal transmission from cell surface cyclic AMP receptors to intracellular events concerned with chemotaxis involves inositol 1,4,5-trisphosphate (1,4,5-IP3): micromolar amounts of 1,4,5-IP3 or Ca2+ were found to mimic the effects of chemoattractants and 1,4,5-IP3 triggered release of Ca2+ from non-mitochondrial stores. Here we report a ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1987
ISSN: 0014-5793
DOI: 10.1016/0014-5793(87)80848-7